Organizational principles of active chromatin at transcription condensates

Many biomolecular condensates associate with specific chromatin loci in the cell nucleus. Transcription condensates are linked to super-enhancer chromatin domains that are characterized by extended accumulations of transcription factor and coactivator binding sites as well as stereotypic epigenetic marks. Bulk assays suggest that these chromatin marks are required to maintain transcription condensates at specific chromatin loci. We use multi-color super-resolution microscopy to investigate the spatial organization of chromatin and transcription condensates. Our results indicate an enrichment of active chromatin at the condensate surface. We discuss implications for the mechanical interaction between condensates and chromatin as well as structure-function relationships of transcription condensates.