An Optics Investigation to Differentiate between Reflection and Fluorescence an Introductory Physics Course

Understanding spectra and the interactions of photons and matter can be important for students of the life sciences. For example, fluorescent dyes are now commonly used in biological applications to improve contrast or highlight the action of specific molecules. Many introductory physics courses limit their discussion of optics to refraction in prisms or image for mation by thin lenses. This poster presents an introductory physics lab activity in which students use an array of different pigments, multiple colored high-intensity flashlights, and a Vernier Spectrometer to differentiate between reflection and flurescence spectra.