5-Aminolevulinic Acid-Based(5-Ala) in the Treatment of Genetic Disorder Using Photodynamic Therapy

5-Aminolevulinic acid (5-ALA) is a non-proteinogenic amino acid utilized notably in photodynamic therapy (PDT) to treat various diseases, including genetic disorders and cancers.

This treatment modality combines a photosensitizing agent, light, and oxygen to produce a cytotoxic effect primarily localized to the target tissue. Within the target cells, 5-ALA is metabolized to Protoporphyrin IX (PpIX), a potent photosensitizer. Upon exposure to light of a specific wavelength, PpIX produces reactive oxygen species (ROS), leading to cell damage and death. This light exposure is carefully controlled and localized, minimizing damage to surrounding healthy tissue.

This study elucidates the molecular mechanisms, electronic structure, and reactivity of PpIX, providing insights crucial for optimizing its therapeutic efficacy. Spectroscopy on PpIX and its isomers was obtained to determine absorption, fluorescence, and phosphorescence characteristics. Also, DFT, which is a quantum mechanical modeling method, was used to investigate the electronic structure. Energy levels, molecular orbitals, and electronic density of the molecules of PpIX and isomers were observed and analyzed. While primarily known for its role in treating diseases by PDT, the potential of 5-ALA-based photodynamic therapy in managing genetic disorders offers an intriguing avenue for research and clinical application, warranting a more thorough investigation.