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Carbon-13 NMR spectroscopic tracking of sugar metabolism in cultured SfXL glioblastoma cells over long incubation time periods

ORAL

Abstract

Glioblastoma, the cancer of the cerebral glial cells, is an aggressive type of tumor that is known to be addicted to sugars and exhibit Warburg effect. In this study, we have investigated via carbon-13 ($^{\mathrm{13}}$C) NMR spectroscopy the long-term metabolism of sugars such as glucose in cultured SfXL glioblastoma cells over a four-day period of incubation times. To achieve this, eight flasks of glioblastoma cells were cultured, four were doped with 10mM uniformly labeled $^{\mathrm{13}}$C glucose and four were doped with 10mM unlabeled glucose. The flasks were then left to incubate for a set time period. One $^{\mathrm{13}}$C glucose vial and one unlabeled glucose vial were then harvested at 24-hours, another pair at 48-hours, another at 72-hours, and the final pair at 96-hours. Both media and cell extracts of each flask were then processed for $^{\mathrm{13}}$C NMR analyses. The NMR data showed that lactic acid increased significantly throughout the 96-hour period, and secondary metabolites such as acetate and alanine also increased. The details of the $^{\mathrm{13}}$C NMR results for glucose metabolism as well as those of fructose will be presented here. This study is supported by the Welch Foundation grant AT-1877, DOD grants W81XWH-21-1-0176 and W81XWH-19-1-0741, CPRIT grant RP180716, and the UTD CoBRA and SPIRE grants.

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Authors

  • Haley Sachse

    University of Texas at Dallas

  • Chao Ma

    University of Texas at Arlington, Florida International University, Texas A&M University-Commerce, Texas A&M University–Commerce, University of Houston Downtown, Texas A\&M University, Carnegie Mellon University, Department of Physics, MSEC, Texas State University, Ingram School of Engineering, MSEC, Texas State University, MSEC, Texas State University, Ingram School of Engineering, Texas State University, Department of Chemistry and Biochemistry, North Carolina Central University, Durham, NC 27707, USA, Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802, USA, Texas Tech University, Texas A&M University, University of San Francisco, University of Wuppertal, University of Illinois at Urbana-Champaign, University of Houston, University of Texas at Dallas, Mitchell Institute for Fundamental Physics and Astronomy, Texas A & M University, Center for Neutrino Physics, Department of Physics Virginia Tech, None, Department of Physics, University of Texas at Dallas, Department of Electrical Engineering, Yale University

  • Chao Ma

    University of Texas at Arlington, Florida International University, Texas A&M University-Commerce, Texas A&M University–Commerce, University of Houston Downtown, Texas A\&M University, Carnegie Mellon University, Department of Physics, MSEC, Texas State University, Ingram School of Engineering, MSEC, Texas State University, MSEC, Texas State University, Ingram School of Engineering, Texas State University, Department of Chemistry and Biochemistry, North Carolina Central University, Durham, NC 27707, USA, Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802, USA, Texas Tech University, Texas A&M University, University of San Francisco, University of Wuppertal, University of Illinois at Urbana-Champaign, University of Houston, University of Texas at Dallas, Mitchell Institute for Fundamental Physics and Astronomy, Texas A & M University, Center for Neutrino Physics, Department of Physics Virginia Tech, None, Department of Physics, University of Texas at Dallas, Department of Electrical Engineering, Yale University