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Carbon-13 NMR Spectroscopy of Aberrant Beta-Galactosidase and Arginase Activities in Cancer$\backslash $cf0

ORAL

Abstract

h $-abstract-$\backslash $pard Hyperdrive metabolism is a common occurrence in cancer as more nutrients and raw materials are required to sustain rapid proliferation and growth, non-invasive detection methods for assessing such abnormal metabolic activities could potentially provide definitive diagnostic information of said disease. In this study, we have investigated the feasibility of carbon-13 labeled lactose ureide and arginine as potential biomarkers for the early detection of cancer. In particular, we used 13C nuclear magnetic resonance (NMR) spectroscopy to track the metabolism of two human cancer cell lines with 13C-lactose ureide and 13C-arginine, respectively: 1) 13C-lactose ureide in living MCF-7 breast cancer cells which have upregulated beta-galactosidase activity, and 2) 13C-arginine metabolism in HuH7 hepatocarcinoma cells. NMR data on the metabolic results will be discussed in view of their potential as non-invasive, in-vivo biomarkers for cancer. This study is supported by the Welch Foundation grant AT-1877-20180324, DOD grants W18XWH-17-1-0303 and W81XWH-19-1-0741, Cancer Prevention and Research Institute of Texas (CPRIT) grant RP180716, and the UTD Collaborative Biomedical Research Award (CoBRA).$\backslash $pard-/abstract-$\backslash $\tex

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Authors

  • Chelsea Sanchez

    Department of Physics, University of Texas at Dallas, Richardson, TX 75080., University of Texas at Dallas

  • Eric Davis

    University of Texas at Arlington, Sultan Qaboos University, UTA HEP group, Department of Integrated Bio \& Nano Systems, University of Houston, Houston, TX 77204, USA, Department of Electrical and Computer Engineering, Nano Tech Center, Texas Tech University, University of Michigan, University of Waerloo, Canada, Texas Tech University, Dept of Mechanical Eng, TTU; Dept. of Physics and Astronomy, TTU, Dept of Cell Biology and Biochemistry, TTU Health Sci Ctr, Dept of Physics and Astronomy, TTU, Dept of Mechanical Engineering, TTU, Dept of Physics and Astronomy, TTU; Sch of Health and Sci, Purdue Univ, Dept of Mathematical and Systems Engineering, Shizuoka Univ, Department of Physics, University of Texas at Dallas, Richardson, TX 75080, Department of Physics, University of Texas at Dallas, Richardson, TX 75080., LeTourneau University, None, University of Waterloo, Canada, Texas Tech Univ, Trinity University, the University of Texas at Dallas, Texas Tech University, Lubbock, TX, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada, University of Dallas, Liverpool John Moores University, University Of Houston, Biolog Department, TCU, Home Schooled high school student, Los Alamos National Laboratory

  • Eric Davis

    University of Texas at Arlington, Sultan Qaboos University, UTA HEP group, Department of Integrated Bio \& Nano Systems, University of Houston, Houston, TX 77204, USA, Department of Electrical and Computer Engineering, Nano Tech Center, Texas Tech University, University of Michigan, University of Waerloo, Canada, Texas Tech University, Dept of Mechanical Eng, TTU; Dept. of Physics and Astronomy, TTU, Dept of Cell Biology and Biochemistry, TTU Health Sci Ctr, Dept of Physics and Astronomy, TTU, Dept of Mechanical Engineering, TTU, Dept of Physics and Astronomy, TTU; Sch of Health and Sci, Purdue Univ, Dept of Mathematical and Systems Engineering, Shizuoka Univ, Department of Physics, University of Texas at Dallas, Richardson, TX 75080, Department of Physics, University of Texas at Dallas, Richardson, TX 75080., LeTourneau University, None, University of Waterloo, Canada, Texas Tech Univ, Trinity University, the University of Texas at Dallas, Texas Tech University, Lubbock, TX, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada, University of Dallas, Liverpool John Moores University, University Of Houston, Biolog Department, TCU, Home Schooled high school student, Los Alamos National Laboratory

  • Eric Davis

    University of Texas at Arlington, Sultan Qaboos University, UTA HEP group, Department of Integrated Bio \& Nano Systems, University of Houston, Houston, TX 77204, USA, Department of Electrical and Computer Engineering, Nano Tech Center, Texas Tech University, University of Michigan, University of Waerloo, Canada, Texas Tech University, Dept of Mechanical Eng, TTU; Dept. of Physics and Astronomy, TTU, Dept of Cell Biology and Biochemistry, TTU Health Sci Ctr, Dept of Physics and Astronomy, TTU, Dept of Mechanical Engineering, TTU, Dept of Physics and Astronomy, TTU; Sch of Health and Sci, Purdue Univ, Dept of Mathematical and Systems Engineering, Shizuoka Univ, Department of Physics, University of Texas at Dallas, Richardson, TX 75080, Department of Physics, University of Texas at Dallas, Richardson, TX 75080., LeTourneau University, None, University of Waterloo, Canada, Texas Tech Univ, Trinity University, the University of Texas at Dallas, Texas Tech University, Lubbock, TX, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada, University of Dallas, Liverpool John Moores University, University Of Houston, Biolog Department, TCU, Home Schooled high school student, Los Alamos National Laboratory

  • Eric Davis

    University of Texas at Arlington, Sultan Qaboos University, UTA HEP group, Department of Integrated Bio \& Nano Systems, University of Houston, Houston, TX 77204, USA, Department of Electrical and Computer Engineering, Nano Tech Center, Texas Tech University, University of Michigan, University of Waerloo, Canada, Texas Tech University, Dept of Mechanical Eng, TTU; Dept. of Physics and Astronomy, TTU, Dept of Cell Biology and Biochemistry, TTU Health Sci Ctr, Dept of Physics and Astronomy, TTU, Dept of Mechanical Engineering, TTU, Dept of Physics and Astronomy, TTU; Sch of Health and Sci, Purdue Univ, Dept of Mathematical and Systems Engineering, Shizuoka Univ, Department of Physics, University of Texas at Dallas, Richardson, TX 75080, Department of Physics, University of Texas at Dallas, Richardson, TX 75080., LeTourneau University, None, University of Waterloo, Canada, Texas Tech Univ, Trinity University, the University of Texas at Dallas, Texas Tech University, Lubbock, TX, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada, University of Dallas, Liverpool John Moores University, University Of Houston, Biolog Department, TCU, Home Schooled high school student, Los Alamos National Laboratory