Influence of Static Magnetic Fields on Ion Channel Activity and Membrane Potential in HT22 Cells

The interaction between magnetic fields and biological systems, particularly in wound healing, cell growth, and regeneration, is an area of increasing interest. Our lab has shown that 1 mT static magnetic fields enhance HT22 cell proliferation, with the rate depending on the field’s orientation. Downward fields yield the highest proliferation rate, followed by horizontal and upward orientations. Initial findings suggest that this effect is more pronounced in potassium-rich media, likely due to potassium channel modulation. To confirm this hypothesis, we assessed whether similar changes occurred in a cesium-rich media. We further explored the influence of static magnetic fields on intracellular calcium levels and membrane potential using Fura-2 AM and FluoVolt fluorescence indicators dyes, respectively.