Modular Hyperspectral Imaging System

Hyperspectral imaging (HSI) combines spectroscopy with imaging, providing large field-of-view (FOV) and sensitivity to both molecular and morphological properties of samples. Recently multiple different clinical and preclinical applications of HSI have been reported. The commercially available HSI systems lack modularity and adaptability, reducing their applicability in clinical and preclinical environment.



In this contribution, we present development and characterization of a modular push-broom HSI system for imaging samples in transmission and reflection utilizing the 400 – 1000 nm spectral range. To avoid sample heating, a novel multi-LED illumination system was developed. By imaging USAF resolution targets, a maximal spatial resolution of 100 μm was determined. Different objectives resulted in FOV of either 40 mm (resolution 0.1 mm) or 153 mm (resolution 0.3 mm). The spectral resolution was 2.9 nm. Finally, the imaging performance was verified by comparing measured absorption spectra of different diluted dies to a reference spectrograph. The system was equipped with optional modules that provide 3D shape acquisition, thermal and fluorescence imaging.



The adaptability and modularity of the system enables broad uses in biomedical applications and beyond them. As a demonstration we present a set of applications, including in-vivo human hand imaging, imaging of mouse tumors and imaging of human skin bruises.