NMR Spectroscopic Investigation of the Effect of LDH inhibitor Sodium oxamate on Glucose metabolism in cancer cell

Sodium oxamate is an inhibitor of lactate dehydrogenase (LDH) that specifically inhibit LDH-A,

an important enzyme that is active in most of tissues catalyzes the reversible conversion of pyruvate to lactate.

In this work, we have investigated via carbon-13 nuclear magnetic resonance the concentration dependence of sodium oxamate and the role of glutamine on [1-13C] glucose metabolism in a variety of cultured cancers cell including renal cell carcinoma (786-O), hepatocellular carcinoma (HepG-2), and glioblastoma (SfXL cells). Our results reveal that highest concentration of sodium oxamate affect lactate production and other glucose metabolites in these cancer cell. Intra- and extra-cellular metabolic products of glucose will be discussed in light of the key role of glutamine in glucose metabolism. . This study is supported by the Welch Foundation grant AT-1877-20180324, DOD grants W18XWH-17-1-0303 and W81XWH-19-1-0741, Cancer Prevention and Research Institute of Texas (CPRIT) grant RP180716, and the UTD Collaborative Biomedical Research Award (CoBRA)