Effect of charge patterning and hydrophobicity in microrheological properties of peptide-based complex coacervates

Liquid-liquid phase separation is a developing field for understanding intracellular structure, the genesis of life, and disease. The development of new liquid-based organelles and therapeutics may be amplified by addressing the design principles behind the phase separation, but an understanding of how monomer sequence and hydrophobicity affect the physical properties of the material, and consequently, strategies to influence the phase dynamics are inadequate. To understand charge-driven phase separation between polypeptides, microrheology is used to find that the change in viscosity of coacervates. Charge patterning and hydrophobicity are predicted to have a significant impact on viscosity. Overall, these observations offer a fresh mechanism for comprehending the sequence-level components that influence the coacervate dynamics.