Assessing nordihydroguaiaretic acid therapeutic effect for glioblastoma

Raman microscopy combined with computational analysis was used to detect structural changes in glioblastoma (GBM) biosignatures due to nordihydroguaiaretic acid (NDGA) administration. As a potential therapeutic agent, NDGA is a reactive oxygen species (ROS) scavenger and anti-oxidant. This phenolic lignan had positive effects on multi-organ malignant tumor reduction and inhibition. The current analysis of NDGA's effect on GBM human cells shows a decrease in altered protein content and ROS-damaged phenylalanine. The use of phenylalanine as a biomarker for differentiating across samples and evaluating NDGA's effectiveness is a new finding discussed here. The creation of lipid droplets and a decline in the altered protein content indicate that treatment with a low dosage of NDGA over long periods reduces abnormal lipid-protein metabolism. The knowledge acquired via this research is significant for comprehending both the positive and negative bio-effects of NDGA as a potential treatment for brain cancer.