Probing cell-matrix interactions in 3D at the local scale

In this talk, I will introduce our recent works in quantitatively studying how cells interact with their surrounding extracellular matrix. In particular, I will discuss the critical impact of the matrix nonlinear elasticity in regulating cell-ECM mechanical interactions. For example, the nonlinear stiffening nature of the ECM enables a significantly extended stress dissipation, and the creation of a stiff shell surrounding the cell. These effects have an important role in regulating cell-cell communications, as well as the mechanobiology of cell-ECM interactions. In addition, I will also show results revealing the critical role of interfacial curvature on cell migratory behaviors.