Motility analysis of Vibrio alginolyticus after simulated microgravity

Simulated microgravity is a unique fluid environment used to mimic specific characteristics of the microgravity environment including low fluid shear, low turbulence, and lack of sedimentation. Different devices have been created to simulate microgravity, one of which is the high aspect ratio vessel (HARV) and the tool of this work. Vibrio alginolyticus, a marine pathogen, was used in this study to examine the impact of the simulated microgravity environment (HARV) on Vibrio’s motility. Vibrio’s motility has been characterized as a run-reverse-flick motility pattern. We used digital holographic microscopy (DHM) to record holograms that contain XYZT information and analyzed the recordings. Through 3D processing and analysis, we show that Vibrio’s motility patterns as described in other sources do not appear altered. Characterizing and understanding changes to Vibrio’s motility as a result of this environment are important as motility plays a key role in their chemotaxis and potentially virulence.