Nanopore genomic mapping using single-strand binding protein

Nanopore sequencing measures the electrical signal associated with the translocation of long strands of DNA through a nanoscale pore. Base-level sequencing is possible with biological pores, but long times and high parallelization are required compared to solid-state nanopores. Rapid genomic mapping technologies have been developed that image the AT content of DNA molecules confined in nanochannels. Here, we develop an assay to combine the speed of nanochannel mapping with the portability of solid-state nanopores for rapid point-of-care genomics. We heat DNA to the sequence-dependent denaturation point of it's AT bonds and introduce a single-strand binding protein (SSB) that will bind the open AT bond sites. This binding protein provides a large blockade signal as it translocates through the nanopore, allowing us to map the DNA and create a barcode of AT density along the molecule. Here, we present preliminary results from standard reference genomes and discuss the physics of DNA-SSB complexes.