1D and 2D nanostructure formation via click conjugation of coiled-coil peptide bundlemer building blocks

Alpha-helical peptides that themselves assemble into coiled-coil bundles, or bundlemers, are being explored as a versatile building block to construct well-defined nanostructures. Computational designed 29 amino acids peptides self-assemble into monodisperse, homotetrameric coiled coils that are antiparallel in the water. With the tunable bundlemer surface groups and peptide termini, covalent 'click' conjugation reactive groups can be precisely presented for desired inter-bundlemer covalent interaction. Building blocks have been linked together to construct a 1D physical-covalent hybrid supramolecular polymer as previously shown. Herein we report our novel design of peptide bundlemer with click functional group presented from the side of the coiled-coil bundle. Novel ladder-like 1D peptide nanostructures are targeted by clicking the end-functionalized bundlemers with the new body-functionalized peptide bundlemer. In addition, we have also shown the capability to extend this 1D ladder-like peptide nanostructure to a 2D peptide sheet by controlling the surface functional groups on the coiled-coil peptide bundlemers.