High Resolution Imaging through Structured Illumination in Lens-less Incoherent Microscopy

Lens-less microscopy using sinusoidal structured illumination to attain high resolution and large field of view is the main target of this project. The schematic includes an LED,followed by a Spatial Light Modulator,a USAF resolution chart,and CMOS sensor respectively.Structured Illumination is carried out by using an incoherent light,LED,as illumination source.The illumination light is modulated sinusoidally by using a Spatial Light Modulator.Sinusoidal illumination pattern having cosine waves with three different phase shifts is generated in MATLAB and then assigned to SLM to downmodulate higher spatial frequencies.The sample information is recorded on sensor without using any objective lens.To retrieve the sample information,from captured raw images corresponding to each phase shift,backward propagation model is applied using MATLAB.Phase and amplitude information of sample is obtained from the final reconstructed image.The project aims to design a lens-less microscope with high resolution,portability,large field of view and depth of field,compared with conventional microscopes.This technique has many common applications in biomedical engineering for cellular studies.In future,lens-less microscopy might replace optical microscopy due to its simplicity and cost-effectiveness.