Optical probing of light localization properties to quantify the probiotic effect on chronic alcoholic brain cells via confocal imaging

The quantification of photonics localization is an important technique to study the molecular specific structural abnormalities. Chronic alcoholism results in medical, behavioral, and psychological problems which initially damages the brain at the cellular level. While the probiotic treatment helps in retrieving the physical damage to the alcoholic’s brain. The effects of probiotic Lactobacillus Plantarum treatment on chronic alcoholic brain cells/chromatin using the inverse participation ratio (IPR) a technique in a mouse model is reported. We construct optical lattices based on the confocal images and quantify the degree of structural disorder (L_d). The astrocytes, and microglial cells, and chromatin of brain cells are probed for molecular specific overexpression stained with appropriate dyes/proteins. The alcohol-treated glial cells and chromatin have an increase in the L_d which might be due to an adverse effect of alcohol. However, the decreases in the L_d of brain cells and chromatin when fed with alcohol and probiotics simultaneously confirm that probiotics soothe the brain and help to reduce the structural abnormalities. The potential application of this confocal-IPR technique to diagnosing alcohol’s effect and probiotic treatment in chronic alcoholism will be discussed.