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Microfluidics & Algorithm for Comprehensive Small Volume Blood Diagnostics via Rapid Solidification of μL Drops into Homogeneous Thin Film Solid Films and XRF

ORAL

Abstract

Conventional Blood Diagnostics (BD) uses 7- 10 mL of liquid blood and takes hours to days for results. Such blood volumes lead to a 74% rate of ‘Hospital Acquired Anemia’, a condition aggravating chronic illness in the elderly, infants, children, and the critically-ill.

Using microscopy, Ion Beam Analysis, and X-Ray Fluorescence (XRF), the present work studies blood drop microfluidics and rapid blood solidification. The hyper-hydrophilic coating, HemaDrop™, solidifies 10 µL-sized blood drops into Homogeneous Thin Solid Films (HTSFs). A new blood collection and solidification device, InnovaStrip™ [1], allows HTSFs to be analyzed via solid-state techniques for electrolytes and metals composition to ± 10%.

To address issues in XRF automated software, including background fit errors, Fast Accurate Blood Analysis (FABA), a new XRF algorithm specific to blood analysis, is implemented in a mobile app, Fast Hand-held Analysis for XRF. FABA makes BD portable when paired with Hand-Held XRF. It allows for data conversion from atomic % composition into mg/dL using built-in calibration HTSFs, integrated into the InnovaStrip™ design. FABA yields comprehensive BD using μLs of blood with accuracy and reproducibility to ± 10%.

[1] Herbots et al. Int. US. Pat. Pend (2020).

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Presenters

  • Thilina Balasooriya

    Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

Authors

  • Thilina Balasooriya

    Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

  • Wesley Peng

    Physics, Arizona State University, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

  • Nikhil Suresh

    Physics, Arizona State University, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

  • Aashi R Gurijala

    Physics, Arizona State University, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

  • Mohammed Sahal

    Physics, Arizona State University, SiO2 Innovates LLC, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, SiO2 Innovates, LLC

  • Eric Culbertson

    Physics and Eyring Materials Center, Arizona State University

  • Robert J Culbertson

    Physics, Arizona State University, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University

  • Nicole Herbots

    Physics, Arizona State University, Dpt of Physics/Eyring Materials Center, Arizona State University, Physics Dpt/Eyring Materials Cr, Arizona State University, Physics and Eyring Materials Center, Arizona State University