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Effects of rapid impact loading on neural progenitor cells

ORAL

Abstract

We recently developed a high throughput microfluidic MEMS device, the μHammer, to subject individual cells to rapid impact loading, at strains of up to 40% over typical impact durations of ~10 μs. With the μHammer, we can subject >100,000 cells to controlled impacts per experiment, allowing measurement of both single cell properties and statistically-relevant population averages. Cells are collected post-impact and cultured, allowing the time course of damage and recovery to be determined through subsequent analysis. Here, we report the range of cytoskeletal structures and dynamics observed in neural progenitor cells (NPC) before and after sub-lethal impacts, as assessed by high-resolution confocal microscopy. These studies establish important baseline properties of the NPC cytoskeleton, while providing insight into the effects of traumatic injuries on cells and tissues.

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Presenters

  • Megan Valentine

    University of California, Santa Barbara, Mechanical Engineering, University of California, Santa Barbara, Department of Mechanical Engineering, University of California at Santa Barbara, Department of Mechanical Engineering, University of California, Santa Barbara

Authors

  • Delany Rodriguez

    University of California, Santa Barbara, Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara

  • Luke H. C. Patterson

    University of California, Santa Barbara

  • Jennifer L. Walker

    University of California, Santa Barbara

  • Evelyn Rodriguez-Mesa

    Owl Biomedical

  • Kevin Shields

    Owl Biomedical

  • John S. Foster

    Owl Biomedical

  • Adele M. Doyle

    University of California, Santa Barbara

  • Kimberly L. Foster

    Tulane University

  • Megan Valentine

    University of California, Santa Barbara, Mechanical Engineering, University of California, Santa Barbara, Department of Mechanical Engineering, University of California at Santa Barbara, Department of Mechanical Engineering, University of California, Santa Barbara