Effect of pH on the phase behavior of multiple proteins in oppositely charged weak polyelectrolyte solution

We discuss the effect of pH on the phase behavior of weakly dissociating proteins in the presence of oppositely charged polyelectrolytes. We have used a hybrid methodology of coarse-grained single chain mean field simulation with constant pH method in a semi-grand canonical framework to include the fluctuating charge of the proteins and polyelectrolytes. Using the characteristics of the resulting phase behavior pertaining to multiple proteins and several polyelectrolytes, we compare the results due to charge regulation and equivalent fixed charge distribution on proteins.