Doppler Spectroscopy of Intracellular Dynamics During Chemotherapy in Tumor Biopsies

Biodynamic imaging is a high-content optical imaging technology based on Doppler spectroscopy and digital holography that uses dynamic speckle as high-content image contrast to probe living tissue. Biomarkers from the living tissues were extracted using fluctuation spectroscopy from intracellular Doppler light scattering in response to the molecular mechanisms of action of therapeutic drugs that modify a range of intracellular motions. Biodynamic imaging measurements of canine B-cell cancer tissues with unknown outcomes were performed and the drug-response spectrograms were compared to results from tissues of 22 pre-clinical trial dogs with known outcomes. A machine learning classifier was constructed based on feature vector correlations and linear separability in high dimensional feature space. The prediction of resistance or sensitivity to chemotherapy for unknown patient clinical outcomes was demonstrated. These results point to the potential for biodynamic profiling to contribute to personalized medicine by aiding the selection of chemotherapy for cancer patients.