Holographic Characterization of Imperfect Spheres

Holographic snapshots of colloidal spheres can be fit to Lorenz-Mie theory, yielding the radius, refractive index and position of individual colloids in situ. This procedure assumes that the scatterer is a uniformly dense ideal sphere. Via experimentation and simulation, we demonstrate that small deviations from ideal sphericity produce palatable errors (approximately 1{\%}) in our estimation of the particle's physical properties.