Wide two-photon in vivo imaging deep inside thick biological tissue

POSTER

Abstract

Wide 3D imaging deep inside thick intact tissue is a grand challenge. Here we develop a method combining temporal focusing with modulating the spatial degrees of freedom of the incident light. By modulating the phase contrast in ways informed by random matrix theory, compression of spatial distortion and chirped ultrafast pulses through thick biological tissues are anticipated. This method is anticipated to provide a functional platform to map neuron behavior and networks.

Authors

  • Kai Lou

    Departments of Materials Science and Engineering, University of Illinois

  • Bo Wang

    Departments of Materials Science and Engineering, University of Illinois

  • Steve Granick

    Univ of Illinois - Urbana, University of Illinois at Champaign-Urbana, University of Illinois, Urbana-Champaign, University of Illinois Urbana-Champaign, University of Illinois at Urbana-Champaign, Departments of Materials Science and Engineering, Chemistry, Physics, University of Illinois