High-speed Membrane Imaging with Digital Holography

ORAL

Abstract

Lipid membranes can change on timescales faster than traditional three dimensional imaging tools can follow. Digital holography offers a the potential to observe observe membranes in 3D at 1000 Hz or greater to resolve dynamics down to thermal fluctuations. This works because holography encodes 3D information into a single 2D image, allowing imaging limited only by camera speed. However, precise quantitative interpretation of holograms has proved challenging for samples of any complexity. To address this limitation, I am developing methods based on the discrete dipole approximation and a new mathematical approach to solving inverse problems. I will present these methods and preliminary measurements of membrane dynamics using holography.

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Authors

  • Thomas G. Dimiduk

    Harvard, Harvard University, Dept. of Physics

  • Amy Chen

    Harvard

  • Laura Arriaga

    Harvard

  • Vinothan N. Manoharan

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA, Harvard SEAS and Physics, Harvard University, Physics Department and SEAS Harvard University, Harvard University, School of Engineering and Applied Sciences and the Department of Physics, Harvard, Harvard University, School of Engineering and Applied Sciences and Dept. of Physics