Experimental \textit{In Vitro} Test of Differential `Femton' Oximetry (`DFO') Principle for Noninvasive Quantitative Diagnosis of Hypoxia

We report a high-resolution measurement of feasibility of noninvasive diagnosis of hypoxia \textit{via} difference in $\gamma $ rates from n + O --$>$ O + $\gamma $ + n between tumor, O$_{1}$, and normal tissue, O$_{2, }$using $\lambda _{DB }\approx $ 1 fm neutrons (`femtons'), which `count' atoms unaffected by molecular bonds. Q = (O$_{1 }$-O$_{2})$/O$_{1}$ quantifies hypoxia ($<$0), oxia ($>$0), and healthy tissue (=0). Hypoxic breast/prostate tumors have -0.80$>$ Q $<$-0.96. DNA nucleotides dAdenosine, dCytosine and Thymidine, differing by 1 O atom, were irradiated with 14 MeV n's. We observed 1 atom as 0.012 $\pm $ .004 (3$\sigma ) \quad \gamma $ rate difference. This implies that DFO would diagnose \textit{stand alone} hypoxia Q = -0.10 and -0.25 with specificity 95{\%} and 99{\%}, respectively. As benchmark, we measured relative genome lengths of 2 mammal tissues to be Q = -0.12 $\pm $ .02, vs. 0.1 expected. These data suggest Femton Onco Physics as path to needle-less biopsy.