Effect of Hydrophobicity on the Adsorption of Human IgG to Thiolated Gold Surfaces as Characterized by SPR

In order to enhance our understanding of the role of hydrophobicity in nonspecific human immunoglobulin G [hIgG] surface interactions, we studied the adsorption of hIgG to thiol self assembled monolayers [SAMs] on gold using surface plasmon resonance [SPR]. The hydrophilic and hydrophobic SAMs had hydroxyl and methyl terminal groups respectively. Protein adsorption to thiolated surfaces was measured looking at relative SPR resonance angles.