Sum frequency generation microscopy for imaging chirality

ORAL

Abstract

Optically active sum frequency generation microscopy was demonstrated for the first time. Using films of solution of chiral 1,1'-bi-naphthol molecules with microstructures, we showed that sum frequency generation microscopy can image molecular chirality with three-dimensional sectioning capability and sub-micron spatial resolution. Because the image contrast originates from the intrinsic chirality of the system, no staining is required. Our microscopy scheme also allows the simultaneous detection of two-photon fluorescence. As a result, optically active sum frequency generation microscopy can be potentially very useful for live cell imaging. Preliminary results of applying this technique to DNA conformation study will also be described.

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Authors

  • Na Ji

    Department of Physics, University of California, Berkeley

  • Kai Zhang

  • Haw Yang

    Department of Chemistry, University of California, Berkeley, Department of Chemistry, University of California Berkeley, University of California Berkeley, University of California at Berkeley, Department of Chemistry, University of California, Physical Biosciences Division, Lawrence Berkeley National Laboratory

  • Yuen-Ron Shen

    Department of Physics, University of California, Berkeley, Physics Department, University of California at Berkeley, University of California at Berkeley