Numerical modeling on cell death induction by low-temperature plasma

We propose a time-dependent 0D numerical modeling on apoptosis induction by low-temperature plasma, which involves activation of death-receptors, mitochondrial signaling cascade pathway including the disruption of mitochondrial membrane potential, initiator- and executor-caspases and regulators. The effects of plasma irradiation can be modelled as exogenous or endogenous influence. The biochemical system is kinetically solved through a time-dependent 0D numerical simulation involving about 100 agents and about 300 reactions. Our simulation suggests that mitochondrial membrane potential drops due to tBid-(Bax)2 generated by exogenous plasma effects, which in turn results in decreased ATP and released cytochrome c. We shows a time trace of concentration of activated caspase3 at the different H₂O₂ influx during the first 30 minutes. We suggest that the larger H₂O₂ dose causes the faster activation of caspase 3, which in turn results in the apoptosis.