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Comprehensive analysis of gene expression in PAL-treated glioblastoma cells

POSTER

Abstract

We have previously developed plasma-activated medium (PAM) and plasma-activated Ringer’s lactate solution (PAL). PAM induced more intracellular reactive oxygen species (ROS) than PAL did on glioblastoma cells. Gene expression analysis using microarray revealed that PAM induced oxidative-stress dependent cell death on glioblastoma cells. In this study, we have performed microarray analysis of PAL-treated glioblastoma cells. Many histone cluster genes were upregulated more than 2-fold in PAL-treated glioblastoma cells. Histones play a critical role in epigenetic regulation, and histone genes are usually expressed in the S phase (synthesis phase) of cell cycle. Flow cytometry analysis suggest that PAL induced cell cycle arrest on the G2/M and S phases on glioblastoma cells. These results suggest that PAL upregulated histone genes because it induced cell cycle arrest on the S phase on glioblastoma cells.

Publication: [1] H. Tanaka, S. Bekeschus, D. Yan, M. Hori, M. Keidar, M. Laroussi, Plasma-Treated Solutions (PTS) in Cancer Therapy, Cancers, 13 (2021) 1737.<br>[2] H. Tanaka, M. Mizuno, K. Ishikawa, S. Toyokuni, H. Kajiyama, F. Kikkawa, M. Hori, Molecular mechanisms of non-thermal plasma-induced effects in cancer cells, Biol Chem, 400 (2018) 87-91.<br>[3] H. Tanaka, M. Mizuno, K. Ishikawa, S. Toyokuni, H. Kajiyama, F. Kikkawa, M. Hori, New hopes for plasma-based cancer treatment, Plasma, 1 (2018) 150-155.<br>[4] H. Tanaka, K. Ishikawa, M. Mizuno, S. Toyokuni, H. Kajiyama, F. Kikkawa, H.R. Metelmann, M. Hori, State of the art in medical applications using non-thermal atmospheric pressure plasma, Rev. Mod. Plasma Phys. , 1 (2017) 1-89.<br>[5] H. Tanaka, M. Mizuno, K. Ishikawa, K. Takeda, K. Nakamura, F. Utsumi, H. Kajiyama, H. Kano, Y. Okazaki, S. Toyokuni, S. Maruyama, F. Kikkawa, M. Hori, Plasma Medical Science for Cancer Therapy: Toward Cancer Therapy Using Nonthermal Atmospheric Pressure Plasma, Ieee Transactions on Plasma Science, 42 (2014) 3760-3764.<br>[6] H. Tanaka, M. Mizuno, K. Ishikawa, K. Nakamura, H. Kajiyama, H. Kano, F. Kikkawa, M. Hori, Plasma-Activated Medium Selectively Kills Glioblastoma Brain Tumor Cells by Down-Regulating a Survival Signaling Molecule, AKT Kinase, Plasma Medicine, 1 (2013) 265-277.<br>[7] H. Tanaka, K. Nakamura, M. Mizuno, K. Ishikawa, K. Takeda, H. Kajiyama, F. Utsumi, F. Kikkawa, M. Hori, Non-thermal atmospheric pressure plasma activates lactate in Ringer's solution for anti-tumor effects, Sci Rep, 6 (2016) 36282.<br>[8] H. Tanaka, M. Mizuno, Y. Katsumata, K. Ishikawa, H. Kondo, H. Hashizume, Y. Okazaki, S. Toyokuni, K. Nakamura, N. Yoshikawa, H. Kajiyama, F. Kikkawa, M. Hori, Oxidative stress-dependent and -independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions, Sci Rep, 9 (2019) 13657.<br>[9] H. Tanaka, Y. Hosoi, K. Ishikawa, J. Yoshitake, T. Shibata, K. Uchida, H. Hashizume, M. Mizuno, Y. Okazaki, S. Toyokuni, K. Nakamura, H. Kajiyama, F. Kikkawa, M. Hori, Low temperature plasma irradiation products of sodium lactate solution that induce cell death on U251SP glioblastoma cells were identified, Sci Rep, 11 (2021) 18488.<br><br>

Presenters

  • Hiromasa Tanaka

    Nagoya University, Nagoya Univ

Authors

  • Hiromasa Tanaka

    Nagoya University, Nagoya Univ

  • Masaaki Mizuno

    Nagoya Univ, Nagoya University

  • Ayako Tanaka

    Nagoya Univ

  • Yuki Shibata

    Nagoya Univ

  • Kenji Ishikawa

    Nagoya Univ, Nagoya University, Nagoya University, Japan

  • Hiroki Kondo

    Nagoya Univ, Nagoya University, Japan

  • Hiroshi Hashizume

    Nagoya Univ

  • Camelia Miron

    Nagoya Univ

  • Yasumasa Okazaki

    Nagoya Univ

  • Shinya Toyokuni

    Nagoya Univ, Nagoya University

  • Kae Nakamura

    Nagoya Univ

  • Hiroaki Kajiyama

    Nagoya Univ

  • Fumitaka Kikkawa

    Nagoya Univ

  • Masaru Hori

    Nagoya Univ, Nagoya University, Center for Low-temperature Plasma Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.