Bionanoparticle Characterization Using an Integrated Acoustofluidic Device with Raman Spectroscopy for Vaccine Development

The impact of pandemics and viral outbreaks depends heavily on the speed of the vaccine development. Traditional vaccine production methods involve multiple stages and require manual human intervention, resulting in a long lead time. Developing reliable sensors and measurement methods for automated, real-time monitoring could significantly accelerate this process. Raman spectroscopy is a potential real-time measurement technique for determining the physical characteristics of samples. When dealing with bionanoparticles ranging from 50 nm to 400 nm in diameter, higher concentrations (more than 1E+9 particles/mL) are required. In this presentation, we will develop optimized acoustofluidics, demonstrating the practical application of the acoustofluidic device by successfully measuring Raman spectra from low-concentration Ebola vaccine. Our work offers an approach of real-time bionanoparticle monitoring through microfluidics, enabling the real-time quantification of vaccines.