New Compact Design for A Fourier Lightfield Microscope

New methods for the acquisition and processing of 3D microscopic samples have been in constant development in recent years. An approach of growing interest is Integral Imaging Microscopy or IMic. Recent work has sought to utilize the stereoscopic capabilities of IMic for direction measurements in applied fields such as naval hydrodynamics. This growing demand for IMic, and further plenoptic imaging techniques, has revealed a new need for compact configurations of these systems. In previous work, we demonstrated a ~200cm Fourier Integral Microscope(FIMic) with improved spatial resolution compared to traditional IMic systems by imaging the Fourier plane of an infinity-corrected objective. A new, compact 10cm Fourier Lightfield microscope design is presented, capable of >12μm lateral resolution, a Depth of Field of 0.53mm, and a Field of View of 3.06mm. Successful 3D reconstructions of various scenes captured by the compact imager are presented using the Shift & Multiply reconstruction algorithm in addition to a Richardson-Lucy Deconvolution approach. Further, we demonstrate the reconstructions of photobleached signals in a Low Reynolds number flow via Molecular Tagging Velocimetry.