A multi-focus remote focusing microscope design and publication quality animation techniques for optical designs.

Remote focusing is a relatively recent improvement on fluorescence microscopy allowing for faster imaging times and greater resolution. We present two novel designs for axially swept microscopes with multiple simultaneous foci. In a previous paper, a design was shown which utilized a remote focusing in order to create a focus with variable axial position. Here, we expand upon this design to incorporate multiple focal points and multiple wavelengths. This would allow for the simultaneous imaging of multiple planes, utilizing different wavelengths of light for each plane. Two proof of concepts have been built to verify the efficacy of the designs. One demonstrates the ability to generate parallel laterally scanning foci from a single objective, while the other shows that moving a beam off axis from an objective creates an oblique focus. In addition, we show the results of a new technique for creating animations of optical setups. Using the software Blender, accurately ray traced light paths can be made which respond physically to optical components such as lenses and mirrors. Because of the physically accurate ray tracing, parts can be easily input from CAD software or manufacturers designs and can then be used to construct animations highlighting a design or concept.