Testing Cellular Cultures in Simultaneous Simulated Reduced-Gravity and Radiation Space Environments

POSTER

Abstract

An apparatus was developed to test the effects of space-like reduced-gravity and radiation environments on biological cells. The modified rotary cell culture system (RCCS) has been used to expose mice muscle and skeletal cells to prolonged reduced-gravity and radiation, either separately or simultaneously, to simulate conditions during extended space travel. The apparatus has five cylindrical vessels rotated by a motor driven chain. The cells grown on polystyrene microspheres suspended in a viscous neutral-buoyant fluid within the vessels reach terminal velocity as they fall; the rotation of the vessels prevents the cells from ever settling, inducing a state of constant ``free-fall.'' Viscous drag and centripetal forces counter the effects of gravity and buoyant forces producing very little net force on the cells and thus simulating a low gravity environment. This apparatus is designed specifically for insertion in the USU Space Survivability Test (SST) chamber equipped with a 0.2 to 2.5 MeV beta radiation Sr$^{\mathrm{90}}$ source. Interchangeable graphite shielding produce specific average radiation dose rates cells. The simultaneous rotation of the vessels and exposure to the Sr-90 source provides a terrestrial method to observe space like conditions on cells.

Authors

  • Anya Nielson

    Utah State University

  • John Spence

    Brigham Young University, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, Department of Scientific Research, The Metropolitan Museum of Art, New York, NY 10028, Century Darkroom, Toronto, ON M4M 2S1, Canada, Colorado State University, University of Waterloo, Southern Connecticut State University, Clemson University, Oak Ridge National Laboratory, University of Bordeaux, BYU REU Program, New Mexico State University, Arizona State University, Biodesign Institute, Center for Applied Structural Discovery, University of Utah, University of Hawaii, Johns Hopkins University, Embry-Riddle Aeronautical University, Arizona State University, Utah State University, Department of Physics, United States Air Force Academy, Department of Chemistry, Case Western Reserve University, Air Force Research Laboratory, Wright-Patterson Air Force Base, United States Air Force Academy, Lousiana State University, Brigham Young University - Provo, The University of New Mexico, Department of Physics and Astronomy, Brigham Young University, SLAC National Accelerator Laboratory, Department of Chemistry, Brigham Young University, Department of Materials, Devices, and Energy Technologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA, Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, USA, Center for Memory and Recording research, UCSD, Advanced Photon Source, Argonne National Laboratory, University of New Mexico, Los Alamos National Laboratory, University of Chicago

  • John Spence

    Brigham Young University, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, Department of Scientific Research, The Metropolitan Museum of Art, New York, NY 10028, Century Darkroom, Toronto, ON M4M 2S1, Canada, Colorado State University, University of Waterloo, Southern Connecticut State University, Clemson University, Oak Ridge National Laboratory, University of Bordeaux, BYU REU Program, New Mexico State University, Arizona State University, Biodesign Institute, Center for Applied Structural Discovery, University of Utah, University of Hawaii, Johns Hopkins University, Embry-Riddle Aeronautical University, Arizona State University, Utah State University, Department of Physics, United States Air Force Academy, Department of Chemistry, Case Western Reserve University, Air Force Research Laboratory, Wright-Patterson Air Force Base, United States Air Force Academy, Lousiana State University, Brigham Young University - Provo, The University of New Mexico, Department of Physics and Astronomy, Brigham Young University, SLAC National Accelerator Laboratory, Department of Chemistry, Brigham Young University, Department of Materials, Devices, and Energy Technologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA, Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, USA, Center for Memory and Recording research, UCSD, Advanced Photon Source, Argonne National Laboratory, University of New Mexico, Los Alamos National Laboratory, University of Chicago

  • John Spence

    Brigham Young University, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, Department of Scientific Research, The Metropolitan Museum of Art, New York, NY 10028, Century Darkroom, Toronto, ON M4M 2S1, Canada, Colorado State University, University of Waterloo, Southern Connecticut State University, Clemson University, Oak Ridge National Laboratory, University of Bordeaux, BYU REU Program, New Mexico State University, Arizona State University, Biodesign Institute, Center for Applied Structural Discovery, University of Utah, University of Hawaii, Johns Hopkins University, Embry-Riddle Aeronautical University, Arizona State University, Utah State University, Department of Physics, United States Air Force Academy, Department of Chemistry, Case Western Reserve University, Air Force Research Laboratory, Wright-Patterson Air Force Base, United States Air Force Academy, Lousiana State University, Brigham Young University - Provo, The University of New Mexico, Department of Physics and Astronomy, Brigham Young University, SLAC National Accelerator Laboratory, Department of Chemistry, Brigham Young University, Department of Materials, Devices, and Energy Technologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA, Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, USA, Center for Memory and Recording research, UCSD, Advanced Photon Source, Argonne National Laboratory, University of New Mexico, Los Alamos National Laboratory, University of Chicago

  • John Spence

    Brigham Young University, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, Department of Scientific Research, The Metropolitan Museum of Art, New York, NY 10028, Century Darkroom, Toronto, ON M4M 2S1, Canada, Colorado State University, University of Waterloo, Southern Connecticut State University, Clemson University, Oak Ridge National Laboratory, University of Bordeaux, BYU REU Program, New Mexico State University, Arizona State University, Biodesign Institute, Center for Applied Structural Discovery, University of Utah, University of Hawaii, Johns Hopkins University, Embry-Riddle Aeronautical University, Arizona State University, Utah State University, Department of Physics, United States Air Force Academy, Department of Chemistry, Case Western Reserve University, Air Force Research Laboratory, Wright-Patterson Air Force Base, United States Air Force Academy, Lousiana State University, Brigham Young University - Provo, The University of New Mexico, Department of Physics and Astronomy, Brigham Young University, SLAC National Accelerator Laboratory, Department of Chemistry, Brigham Young University, Department of Materials, Devices, and Energy Technologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA, Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, USA, Center for Memory and Recording research, UCSD, Advanced Photon Source, Argonne National Laboratory, University of New Mexico, Los Alamos National Laboratory, University of Chicago

  • John Spence

    Brigham Young University, Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, Department of Scientific Research, The Metropolitan Museum of Art, New York, NY 10028, Century Darkroom, Toronto, ON M4M 2S1, Canada, Colorado State University, University of Waterloo, Southern Connecticut State University, Clemson University, Oak Ridge National Laboratory, University of Bordeaux, BYU REU Program, New Mexico State University, Arizona State University, Biodesign Institute, Center for Applied Structural Discovery, University of Utah, University of Hawaii, Johns Hopkins University, Embry-Riddle Aeronautical University, Arizona State University, Utah State University, Department of Physics, United States Air Force Academy, Department of Chemistry, Case Western Reserve University, Air Force Research Laboratory, Wright-Patterson Air Force Base, United States Air Force Academy, Lousiana State University, Brigham Young University - Provo, The University of New Mexico, Department of Physics and Astronomy, Brigham Young University, SLAC National Accelerator Laboratory, Department of Chemistry, Brigham Young University, Department of Materials, Devices, and Energy Technologies, Sandia National Laboratories, Albuquerque, New Mexico 87185, USA, Department of Physics, New Mexico State University, Las Cruces, New Mexico 88003, USA, Center for Memory and Recording research, UCSD, Advanced Photon Source, Argonne National Laboratory, University of New Mexico, Los Alamos National Laboratory, University of Chicago