Nucleus dynamics in budding yeast

POSTER

Abstract

Timely nuclear movements are essential during many cellular processes such as during brain development in animals, and for proper chromosome inheritance during growth and development of various fungal species. A complete understanding of the process of nuclear migration requires an understanding of the physical properties of the nucleus and the cytoplasm. The budding yeast \textit{Saccharomyces cerevisiae} makes an ideal platform for research due to their genetic tractability, and ease of imaging. We have imaged the nuclei of yeast cells using confocal microscopy, and analyzed the nuclear motion. Our data show that the nuclei experience periods of subdiffusive confinement with occasional periods of superdiffusive directed motion that are attributed to the effect of molecular motors. Future studies will permit us to develop a quantitative description of the viscous and elastic components of the nucleus and cytoplasm that are responsible for the subdiffusive confinement and the properties of external forces required to induce superdiffusive movement.

Authors

  • Carsten Dietvorst

    Colorado State University, Fort Collins, CO

  • Colin Roberts

    High Precision Devices, Boulder, CO, Raytheon, Tucson, AZ, Seagate Technology, Minneapolis, MN, Ball Aerospace (retired), Bloomfield, CO, New Mexico State University, Brigham Young University, Colorado State University, Heinrich-Heine-Universitat Dusseldorf, National Security Technologies, Universidad de Buenos Aires, Colorado State Univ, Colorado College, Utah State University, Advisor, Material Physics Group, Utah State University, Box Elder Innovations, LLC, JILA and Department of Physics, CU Boulder, JILA and Department of Mathematics, CU Boulder, Colorado State University, Fort Collins, Colorado 80523, USA, JILA, NIST and the University of Colorado, Boulder, NIST, University of Colorado / NIST, University of Colorado/JILA, Colorado Sch of Mines, Colorado School of Mines, Southwestern Indian Polytechnic Institute, UC-Berkeley, Colorado State University, Fort Collins, CO, Wroclaw University of Science and Technology, Wroclaw, Poland, JILA, University of Colorado and NIST, Harvard University and Harvard-MIT Center for Ultracold Atoms, Univ of Colorado - Boulder, USAFA, Univ of Denver, Boyce Research Initiatives and Educational Foundation, Brilliant Sky Observatory, San Diego Mesa College, Utah Valley University, University of Colorado Boulder, Brigham Young Univ - Provo, Oak Ridge National Laboratory, University of Sherbrooke, NIST Boulder, Universidad Complutense de Madrid, Electrical and Computer Engineering Department, Colorado State University, Fort Collins, Co 80525, Lawrence Livermore National Lab. (United States), Physics Department, Colorado State University, Fort Collins, Co 80525, JILA, Department of Physics, University of Colorado Boulder, Los Alamos National Laboratory, University of Alabama, University of Wyoming, University of Guelph, University of Guelph, Canadian Light source

  • Colin Roberts

    High Precision Devices, Boulder, CO, Raytheon, Tucson, AZ, Seagate Technology, Minneapolis, MN, Ball Aerospace (retired), Bloomfield, CO, New Mexico State University, Brigham Young University, Colorado State University, Heinrich-Heine-Universitat Dusseldorf, National Security Technologies, Universidad de Buenos Aires, Colorado State Univ, Colorado College, Utah State University, Advisor, Material Physics Group, Utah State University, Box Elder Innovations, LLC, JILA and Department of Physics, CU Boulder, JILA and Department of Mathematics, CU Boulder, Colorado State University, Fort Collins, Colorado 80523, USA, JILA, NIST and the University of Colorado, Boulder, NIST, University of Colorado / NIST, University of Colorado/JILA, Colorado Sch of Mines, Colorado School of Mines, Southwestern Indian Polytechnic Institute, UC-Berkeley, Colorado State University, Fort Collins, CO, Wroclaw University of Science and Technology, Wroclaw, Poland, JILA, University of Colorado and NIST, Harvard University and Harvard-MIT Center for Ultracold Atoms, Univ of Colorado - Boulder, USAFA, Univ of Denver, Boyce Research Initiatives and Educational Foundation, Brilliant Sky Observatory, San Diego Mesa College, Utah Valley University, University of Colorado Boulder, Brigham Young Univ - Provo, Oak Ridge National Laboratory, University of Sherbrooke, NIST Boulder, Universidad Complutense de Madrid, Electrical and Computer Engineering Department, Colorado State University, Fort Collins, Co 80525, Lawrence Livermore National Lab. (United States), Physics Department, Colorado State University, Fort Collins, Co 80525, JILA, Department of Physics, University of Colorado Boulder, Los Alamos National Laboratory, University of Alabama, University of Wyoming, University of Guelph, University of Guelph, Canadian Light source

  • Colin Roberts

    High Precision Devices, Boulder, CO, Raytheon, Tucson, AZ, Seagate Technology, Minneapolis, MN, Ball Aerospace (retired), Bloomfield, CO, New Mexico State University, Brigham Young University, Colorado State University, Heinrich-Heine-Universitat Dusseldorf, National Security Technologies, Universidad de Buenos Aires, Colorado State Univ, Colorado College, Utah State University, Advisor, Material Physics Group, Utah State University, Box Elder Innovations, LLC, JILA and Department of Physics, CU Boulder, JILA and Department of Mathematics, CU Boulder, Colorado State University, Fort Collins, Colorado 80523, USA, JILA, NIST and the University of Colorado, Boulder, NIST, University of Colorado / NIST, University of Colorado/JILA, Colorado Sch of Mines, Colorado School of Mines, Southwestern Indian Polytechnic Institute, UC-Berkeley, Colorado State University, Fort Collins, CO, Wroclaw University of Science and Technology, Wroclaw, Poland, JILA, University of Colorado and NIST, Harvard University and Harvard-MIT Center for Ultracold Atoms, Univ of Colorado - Boulder, USAFA, Univ of Denver, Boyce Research Initiatives and Educational Foundation, Brilliant Sky Observatory, San Diego Mesa College, Utah Valley University, University of Colorado Boulder, Brigham Young Univ - Provo, Oak Ridge National Laboratory, University of Sherbrooke, NIST Boulder, Universidad Complutense de Madrid, Electrical and Computer Engineering Department, Colorado State University, Fort Collins, Co 80525, Lawrence Livermore National Lab. (United States), Physics Department, Colorado State University, Fort Collins, Co 80525, JILA, Department of Physics, University of Colorado Boulder, Los Alamos National Laboratory, University of Alabama, University of Wyoming, University of Guelph, University of Guelph, Canadian Light source