Quantum Dot Localization with Time Resolved Super-Resolution Tracking Microscopy
POSTER
Abstract
A novel setup provides simultaneous measurement of the photoluminescence decay time and local position of single emitters with 100 ps time resolution. For the method, a pulsed laser excites a fluorophore positioned in the confocal optical probe region. The subsequent photons are collected with a high numerical aperture microscope objective and imaged onto a 2x2 array of optical fibers in the image plane. Each fiber is connected to one of four single photon counting detectors. To regulate the emitter position, a piezoelectric stage actively adjusts its location with proportional-based feedback from the four detector intensities, so the emitter remains in the center of the probe region. The time-dependent emission observed on the four detectors is used to monitor the spatial position of the emitter with approximately 10 nm precision. This study provides a foundation for later work that will investigate the structural basis of energy transfer among nanoparticles in higher order configurations.
Authors
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Megan Dunlap
Department of Chemistry, Colorado State University, Fort Collins, CO 80523
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Duncan Ryan
Department of Physics, Colorado State University, Fort Collins, CO 80523
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Martin Gelfand
Department of Physics, Colorado State University, Colorado State Univ, Department of Physics, Colorado State University, Fort Collins, CO 80523
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Peter Goodwin
Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM 87545
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James Werner
Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, NM 87545
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Alan Van Orden
Dept. of Chemistry, Colorado State University, Fort Collins, CO 80523 and Materials Research Institute, Los Alamos National Laboratory, Los Alamo