Water-encapsulated protein source for x-ray serial crystallography

ORAL

Abstract

A reliable source of micron size water droplets has been constructed for the purpose of delivering water-encapsulated protein for x-ray serial crystallography. A linear stream of droplets of negligible divergence is produced by accelerating a liquid water jet through a high pressure gradient [1] inside a converging gas nozzle. Using a co-flowing gas rather than the nozzle walls to squeeze the liquid jet to smaller diameter eliminates the problem of clogging that has thus far limited the minimum size of Rayleigh nozzle jets [2]. Converging jets tend to align non-spherical particles suspended in the liquid and we are presently investigating the possibility of fiber diffraction from drops of aligned macromolecules produced by this method. [1] Ganan-Calvo, A.M. and A. Barrero, \textit{A novel pneumatic technique to generate steady capillary microjets.} Journal of Aerosol Science, 1999. \textbf{30}(1): p. 117-125 [2] http://arxiv.org/abs/physics/0701129

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Authors

  • D.P. DePonte

    Department of Physics, Arizona State University, Tempe, AZ 85287, USA

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles

  • Seth Putterman

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA, Department of Physics, Arizona State University, Tempe, AZ 85287, USA, College of Optical Sciences, University of Arizona, Colorado State University, University of Virginia, Auburn University, Northern Kentucky University, Utah Valley State College, Brigham Young University, Arizona State University, University of Amsterdam, New Mexico State university, University of Minesota, Pulse Field Facility, NHMFL, LANL, NM, Institute of Physics ASCR , Czech Republic, University of Minesota-Twin City, New Mexico State University, Director, Advanced Photon Source, Argonne National Lab, Australian Synchotron Source, Advanced Photon Source, Argonne National Lab, University of Arizona, U.S. Naval Research Laboratory, Wellesley College, Observatoire de Paris, France, Freie Universitat Berlin, Florida State Univ., Univ. Sci. Tech. China, Iowa State University, Lawrence Livermore National Laboratory, Mahabad Azad University, Carnegie Mellon University, Tempe Preparatory Academy, University of California, Los Angeles